Icariin Promotes Survival, Proliferation, and Differentiation of Neural Stem Cells In Vitro and in a Rat Model of Alzheimer’s Disease

Figure 6

Combination of NSC transplantation and ICA treatment increased the total number of cholinergic neurons in the basal forebrain of FFT rats. (a) Immunofluorescent staining of BrdU (A1, green) and ChAT (A2, red) showed NSCs differentiated into cholinergic neurons in vivo. Scale . (b) Immunofluorescent staining of ChAT in the medial septum (MS) of the ipsilateral and contralateral sides in FFT rats. Scale . (c) Quantitative analysis of the ratio of ChAT(+) neurons in the ipsilateral side compared to contralateral side (intact side) in MS of FFT rats. (d) Immunofluorescent staining of ChAT in the vertical limb of the diagonal band (VDB) of the ipsilateral and contralateral sides in BF of FFT rats. Scale . (e) Quantitative analysis of the ratio of ChAT(+) neurons in the ipsilateral side compared to the contralateral side (intact side) in VDB of FFT rats. Data are expressed as the , . , the FFT model group vs the sham group; , the NSC transplantation group vs the FFT model group; , the group vs the NSC transplantation group. ChAT: choline acetyltransferase (a marker of cholinergic neuron).

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