Stable Long-Term Culture of Human Distal Airway Stem Cells for Transplantation

Figure 1

Bronchoscopic isolation of hDASCs from the patient lung. (a) Schematic illustrating the process of clonogenic hDASC isolation and expansion. (b) Clonogenic cells immunostained with hDASC markers KRT5 and P63. (b1) Bright-field imaging. (b2) Immunofluorescence imaging. Scale bar, 100 μm. (c) Differentiation culture of hDASCs in the monolayer on day 7. (c1) Bright-field imaging. (c2) Immunofluorescence imaging. Scale bar, 50 μm.

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