Stable Long-Term Culture of Human Distal Airway Stem Cells for Transplantation

<div>Expansion of hDASCs under the pharmaceutical-grade condition. (a) Clonogenic cells immunostained with hDASC markers KRT5 and P63 among P4 to P7. Scale bar, 100 <i>μ</i>m. (b) Clonogenic cells immunostained with proliferative markers KI67 among P4 to P7. Scale bar, 100 <i>μ</i>m. (c, d) Immunostaining of indicated AEC1 markers AQP5 and HOPX on monolayer-differentiated DASCs. Scale bar, 100 <i>μ</i>m.</div>

Stem Cells International

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Figure 2

Figure 2: Stable Long-Term Culture of Human Distal Airway Stem Cells for Transplantation 