Stable Long-Term Culture of Human Distal Airway Stem Cells for Transplantation

Figure 4

Evaluation of the tumorigenic potential of hDASCs. (a) Soft agar assay of melanoma and hDASCs. Scale bar, 200 μm. (b) Growth curves of subcutaneous tumors in NOD-SCID mice formed by inoculation with either 10⁷ human embryonic lung fibroblast cell MRC-5, 10⁶ HeLa cells, or 10⁷ hDASCs. The blue arrow indicates that 2 mice of the MRC-5 group were euthanized for gross observation and histopathological examination. The red arrow indicates that 2 mice of the hDASC group were euthanized for gross observation and histopathological examination. Grey arrows indicate that 4 mice and 6 mice of the Hela group were euthanized on day 70 and day 84, respectively, because the longest tumor diameter exceeded 20 mm. in each group. (c) Representative H&E staining of different subcutaneous grafts. Scale bar, 100 μm.

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