Stable Long-Term Culture of Human Distal Airway Stem Cells for Transplantation

Figure 5

Alveolar regeneration by intrapulmonary transplantation of hDASCs. (a) Bright field and direct fluorescent images of the chimeric lung after GFP+ hDASC transplantation. (a1) Mock-transplanted. (a2) Uninjured lung. (a3) GFP-labeling hDASCs transplanted. (b) Immunofluorescence images of the chimeric lung of anti-Lamin A/C (red) with DNA counterstain (DAPI, blue). Scale bar, 1000 μm. (c, d) Immunofluorescence images of the chimeric lung with GFP and human-specific Lamin A/C and hDASC marker KRT5 Abs. Scale bar, 200 μm. (e, f) Immunofluorescence images of the chimeric lung with human-specific Lamin A/C and AEC1 marker AQP5 and RAGE. Scale bar, 30 μm. (g) Immunofluorescence images of the chimeric lung with human-specific Lamin A/C and SCGB1A1. Scale bar, 50 μm.

(a)

(b)

(c)

(d)

(e)

(f)

(g)