mTORC2 Activation Mediated by Mesenchymal Stem Cell-Secreted Hepatocyte Growth Factors for the Recovery of Lipopolysaccharide-Induced Vascular Endothelial Barrier

Figure 1

Effects of MSC-secreted HGF on LPS-induced PMVEC barrier. We used 24 h transwell coculture transwell system for culturing PMVECs with MSC, overexpression HGF of MSC (MSC-HGF), or HGF negative control of MSC (MSC-control). Moreover, PMVECs were treated with HGF (20 ng/ml), with or without stimulation with LPS (100 ng/ml) for 4 h and 24 h. (a) Flow cytometry scatter plot of 4 h VE-cadherin expression with MSC-secreted HGF in LPS-induced PMVECs. (b) Flow cytometry cell counts (%) of 4 h VE-cadherin expression with MSC-secreted HGF in LPS-induced PMVECs. (c) The effect of HGF (20 ng/ml) to LPS-induced PMVEC endothelium lysate tested by western blot with 0.4 and 24 h. (d) VE-cadherin protein expression change with MSC-secreted HGF in LPS-induced PMVECs tested by western blot with 24 h. Results are (). .

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