Vimentin-Rab7a Pathway Mediates the Migration of MSCs and Lead to Therapeutic Effects on ARDS

Figure 4

Rab7a knockout inhibited the migration of MSCs. In order to detect the binding of Rab7a and vimentin, the state of Rab7a contained in vimentin was detected by immunoprecipitation method (a). Lentiviral transfection was used to knock out Rab7a and confirm knockout (b). The scratch test was used to determine the impact of Rab7a knockout on scratch healing, a measure of cell migration capability (c).

(a)

(b)

(c)