hUC-MSCs Attenuate Acute Graft-Versus-Host Disease through Chi3l1 Repression of Th17 Differentiation

Figure 2

Chi3l1 knockdown in hUC-MSCs by Chi3l1-targeting shRNA lentiviral transfection. (a, b) The morphology of Chi3l1 knockdown hUC-MSCs (sh-Chi3l1-MSCs) shown by fluorescence microscopy. Scale bar: 400 μm. (c, d) Oil red O staining was used to analyze the adipogenic ability of sh-Chi3l1-MSCs. (c) Shows the control group, and (d) shows the induced group. (e, f) Alizarin red staining was used to analyze the osteogenesis ability of sh-Chi3l1-MSCs. (e) Is the control group, and (f) is the induced group. Scale bar: 200 μm. (g) Immunophenotypic analysis of sh-Chi3l1-MSCs was performed by flow cytometry. (h) mRNA expression levels of Chi3l1 in sh-Chi3l1-MSCs analyzed by qPCR. (i) Western blotting was used to assess Chi3l1 protein expression in sh-Chi3l1-MSCs. sh-NC: sh-NC-MSCs; sh-Chi3l1: sh-Chi3l1-MSCs.

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