hUC-MSCs Attenuate Acute Graft-Versus-Host Disease through Chi3l1 Repression of Th17 Differentiation

Figure 3

Chi3l1 deletion impairs the therapeutic effects of hUC-MSCs in aGvHD mice. (a–c) Recipient mice (BALB/C) were irradiated with a single dose of 800 cGy total body irradiation (Co⁶⁰γ source) and intravenously injected with C57BL/6J bone marrow cells () plus splenocytes () to induce the aGvHD model. Two days after transplantation, different MSCs, including hUC-MSCs, sh-Chi3l1-MSCs, sh-NC-MSCs (), or PBS, were injected intravenously into aGvHD mice. (a) Survival curves of each group of mice (control: , aGvHD, hUC-MSCs, sh-Chi3l1-MSCs, and sh-NC-MSCs: , log-rank test, ). (b) The clinical score of hUC-MSCs, sh-Chi3l1-MSCs, sh-NC-MSCs, or PBS groups of mice. ; ; ns: not significant. (c) Hematoxylin and eosin staining was used to analyze the histological and pathological changes in the skin, intestine, and lung in aGvHD mice 21 days after hUC-MSCs, sh-Chi3l1-MSCs, sh-NC-MSCs, or PBS treatment. Scale bar: 200 μm, , three independent experiments. CTRL: healthy mice.

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