Nuclear Factor I-C Regulates Stemness Genes and Proliferation of Stem Cells in Various Mineralized Tissue through Epithelial-Mesenchymal Interactions in Dental Epithelial Stem Cells

Figure 3

Loss of Nfic decreases the proliferation of dental epithelial cells. (a) Western blot analyses to evaluate the expression levels of cyclin D1 and p21 in control and Nfic-silenced cells. (b) The proliferation rate of control and Nfic-silenced cells from day 0 to 5. , . (c) Immunohistochemistry (IHC) staining analysis of PCNA-positive cells in the cervical loop of the (a, b) control and (c, d) Nfic-silenced mice. (e) The number of PCNA-positive cells in the cervical loop of these mice after 6 weeks. , . (b) and (d) are higher magnifications of (a) and (c), respectively. Scale bars = ; . Data are presented as the . Abbreviations: DM: dental mesenchyme; IEE: inner enamel epithelium; OEE: outer enamel epithelium; SR: stellate reticulum; TA: transit-amplifying cells; WT: wild type.

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