Nuclear Factor I-C Regulates Stemness Genes and Proliferation of Stem Cells in Various Mineralized Tissue through Epithelial-Mesenchymal Interactions in Dental Epithelial Stem Cells

Figure 5

Nfic regulates Sox2 expression in DPSC (a–e) and BMSC (f–j). (a) Sox2 mRNA and (b) protein expression levels were analyzed in control and Nfic-overexpressing DPSC. , . (c) Sox2 expression in vitro examined in cultured DPSC under fluorescence microscopy. (d) Western blot analyses to evaluate the expression levels of cyclin D1 and p21 in DPSC. (e) Cell population of DPSC from day 0 to 5 with increased Nfic expression. , . (f) Sox2 mRNA and (g) protein expression levels were analyzed in control and Nfic-overexpressing BMSC. , . (h) Sox2 expression in vitro was examined in cultured BMSC under fluorescence microscopy. (i) Western blot analyses to evaluate the expression levels of cyclin D1 and p21 in BMSC. (j) Cell population of BMSC from days 0 to 5 with increased Nfic expression. , . (k) Immunohistochemistry (IHC) analysis of Sox2 expression in mouse femur in vivo. 6 weeks. . Data are presented as the . Abbreviations: BMSC: bone marrow stem cell; DPSC: dental pulp stem cell; IHC: immunohistochemistry; Over: overexpression; WT: wild type.

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