Nuclear Factor I-C Regulates Stemness Genes and Proliferation of Stem Cells in Various Mineralized Tissue through Epithelial-Mesenchymal Interactions in Dental Epithelial Stem Cells

Figure 6

Nfic modulates pluripotency gene expression in mesenchymal stem cells of mineralized tissues. (a) Lin28 and Nanog gene expression levels were analyzed in control, Nfic-overexpressing, and (b) Sox2-overexpressing DPSC. (c) Lin28 and Nanog gene expression levels were analyzed in control, Nfic-overexpressing, and (d) Sox2-overexpressing BMSC. (e) (A–D) The number of ALP-positive colonies of iPSC cultured in OSKM medium compared to the addition of Nfic. (E–H) The number of ALP-positive colonies of iPSC cultured in OKM medium compared to the addition of Nfic. Scale bars = . (f) Quantitative analysis of the number of ALP-positive colonies in various conditioned media. Data are presented as the . Abbreviations: ALP: alkaline phosphatase; BMSC: bone marrow stem cell; Ctrl: control; DPSC: dental pulp stem cell; iPSC: induced pluripotent stem cell; OSKM, Oct4, Sox2, Klf4, c-Myc; OKM, Oct4, Klf4, c-Myc.

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