Nuclear Factor I-C Regulates Stemness Genes and Proliferation of Stem Cells in Various Mineralized Tissue through Epithelial-Mesenchymal Interactions in Dental Epithelial Stem Cells

Figure 7

Nfic does not affect Sox2 expression in soft tissues. (a) Sox2 mRNA and (b) protein expression levels were analyzed in primary cultured fibroblasts harvested from the tongues of Nfic^-/- mice. (c) Immunohistochemistry (IHC) analysis of Sox2 expression in the tongues. (B) and (D) are higher magnifications of boxed (A) and (C). 6 weeks. Scale bars = ; . (d) Proliferation rate of tail, (e) gingival, and (f) dermal fibroblasts from Nfic^-/- mice from day 0 to 5 compared to the WT. , . (g) The number of ALP-positive colonies of iPSC from fibroblasts cultured in OSKM medium compared to the addition of Nfic in a dose-dependent manner. (A), (C), and (E) are higher magnifications of (B), (D), and (F), respectively. (h) Quantitative analysis of the number of ALP-positive colonies in various conditioned media. Data are presented as the . Abbreviations: ALP: alkaline phosphatase; Epi: epithelium; IHC: immunohistochemistry; iPSC: induced pluripotent stem cell; LaPr: lamina propria; OSKM, Oct4, Sox2, Klf4, c-Myc; OKM, Oct4, Klf4, c-Myc; SkMu, skeletal muscle; SqEp: squamous epithelium; WT: wild type.

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