The Efficiency of Direct Maturation: the Comparison of Two hiPSC Differentiation Approaches into Motor Neurons

Figure 2

Morphological characteristics of neurons generated via approaches A and B compared to NPC. (a) A schematic overview of approaches A and B differentiating hiPSC-derived NPC into MNs, created with http://BioRender.com. (b) Representative images of cells of approach A and B captured with the IncuCyte® showing MN-type clusters evolving from homogeneous NPC culture. (c) Neuronal identity was confirmed for both approaches via Bielschowsky silver staining, illustrated by brown-stained nuclei and axons. (d) Immunofluorescent staining for Tubb3 (beta-3-tubulin; red) revealed pronounced neuronal network formation via both approaches. Cell nuclei were counterstained with DAPI. All scale bars, 200 μm.