The Efficiency of Direct Maturation: the Comparison of Two hiPSC Differentiation Approaches into Motor Neurons

Figure 3

Neuronal gene expression pattern of cells of approach A and B. Genes involved in motor neurogenesis (a, c) and genes associated with the expression of neuronal ion channels, neurotransmitters, and neurotrophins (b, d) were differently expressed in approaches A and B compared to NPC, tested by qRT-PCR. Results are shown in a heat map (a, b), where red indicates significant downregulation while green represents significant upregulation of genes, and in a volcano plot (c, d), where a value of 0.05 is indicated by a horizontal red dashed line and a fold regulation of -2.0 and 2.0 is represented by vertical red dashed lines ( independent experiments).

(a)

(b)

(c)

(d)