Characterization of Human-Induced Neural Stem Cells and Derivatives following Transplantation into the Central Nervous System of a Nonhuman Primate and Rats

Figure 1

PBMNCs are reprogrammed into iNSCs. (a) The flowchart is about the procedure converting PBMNCs to iNSCs by using Sendai reprograming vectors expressing the Yamanaka factors. (b) Representative pictures of cells at different time points. Scale bars, 200 μm. (c) A representative picture of the induced iNSCs cultured as a monolayer. Scale bars, 200 μm. (d) A representative picture of the induced iNSCs cultured as spheres. Scale bars, 500 μm. (e) One-week expansion curves of iNSCs of passage nos. 10, 20, and 30. (f) iNSCs stained positive for markers SOX2, N-CADHERIN (NCAD), PAX6, NESTIN, ZO-1, and KI67. Scale bars, 50 μm. (g) Percentages of iNSCs at passage nos. 5, 20, and 40 that were positive for NESTIN, NCAD, KI67, and PAX6, respectively. (h) Karyotype analysis of iNSCs.

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