Characterization of Human-Induced Neural Stem Cells and Derivatives following Transplantation into the Central Nervous System of a Nonhuman Primate and Rats

<div>Differentiation of iNSCs into midbrain dopamine neurons with high efficiency in vitro. (a) Flow chart of the differentiation of iNSCs into DA neurons in vitro. (b) Representative pictures of cells at different differentiation time points. (c) Immunofluorescence staining for NURR1 and FOXA2 on cells of differentiation day 10 (scale bars, 50 <i>μ</i>m) and for TH and GIRK2 on cells of differentiation day 24 (scale bars, 75 <i>μ</i>m). (d) Quantification of positive cells for each marker. (e) Expression of LMX1A, NURR1, FOXA2, TH, and <i>β</i>-actin at the transcription level in iNSC-GFP before differentiation and at differentiation days 10, 13, 18, and 24.</div>

Stem Cells International

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Figure 3: Characterization of Human-Induced Neural Stem Cells and Derivatives following Transplantation into the Central Nervous System of a Nonhuman Primate and Rats 

Figure 3 | Characterization of Human-Induced Neural Stem Cells and Derivatives following Transplantation into the Central Nervous System of a Nonhuman Primate and Rats 