Characterization of Human-Induced Neural Stem Cells and Derivatives following Transplantation into the Central Nervous System of a Nonhuman Primate and Rats

<div>Differentiation of iNSCs into motor neurons in vitro. (a) Flow chart of the differentiation of iNSCs into motor neurons in vitro. (b) Representative pictures of cells at different differentiation time points. (c) Immunofluorescence for OLIG2, HB9, and CHAT on iNSC-GFP-differentiated and embryonic stem cell line H9-differentiated cells at different time points, respectively. Scale bars, 50 <i>μ</i>m. (d) Quantification of differentiated iNSCs positive for OLIG2, HB9, and CHAT at different differentiation time points. (e) Comparison of the efficiencies of motor neuron differentiation from iNSC-GFP versus ESCs (H9 line).</div>

Stem Cells International

fig4

Figure 4

Figure 4: Characterization of Human-Induced Neural Stem Cells and Derivatives following Transplantation into the Central Nervous System of a Nonhuman Primate and Rats 

Figure 4 | Characterization of Human-Induced Neural Stem Cells and Derivatives following Transplantation into the Central Nervous System of a Nonhuman Primate and Rats 