Characterization of Human-Induced Neural Stem Cells and Derivatives following Transplantation into the Central Nervous System of a Nonhuman Primate and Rats

Figure 5

Characterization of cells following engraftment into different brain regions of a nonhuman primate. (a) The schematic diagram of the transplantation experiment. (b) The three brain regions selected for transplantation with region a (motor cortex) engrafted with iNSC-GFP, region b (striatum) engrafted with iNSC-DAPs, and region c (substantia nigra) engrafted with iNSC-DAPs. (c) The GFP-positive cellular grafts were detected at the three engraftment sites three months following transplantation. Scale bars, 1 mm. (d) Three months following transplantation, the engrafted iNSC-GFP at the motor cortex showed the morphology of neurons. Scale bars, 250 μm. (e) Immunofluorescence staining for NURR1, MAP2, TH, and GIRK2 on the transplanted iNSC-DAPs three months following engraftment into the striatum. Scale bars, 50 μm. (f) Three months after transplantation of iNSC-DAPs, quantification of the GFP-positive cells that coexpressed TH, NURR1, GIRK2, and MAP2, respectively. (g) The proportions of TH-positive cells that coexpressed GIRK2, MAP2, and NURR1 three months following transplantation of iNSC-DAPs into the striatum. (h) Immunofluorescence staining for NURR1, MAP2, TH, and GIRK2 three months following engraftment of iNSC-DAPs into the substantia nigra. Scale bars, 100 μm. (i) The proportions of surviving cells that were double-positive for GFP and TH, NURR1, GIRK2, or MAP2, respectively, at the substantia nigra. (j) The proportions of TH-positive cells that coexpressed GIRK2, MAP2, or NURR1 at the substantia nigra.

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