Preclinical Evaluation of the Tumorigenic and Immunomodulatory Properties of Human Bone Marrow Mesenchymal Stromal Cell Populations with Clonal Trisomy 5

Figure 1

Cytogenetic characterization of distinct human MSC populations after ex vivo expansion. (a–d) Karyotyping results of the four investigated MSC cell lines. (a) MSC sample T5-50 karyotype 47,XX,+5. (b) MSC sample T5-53 karyotype 47,XX,+5. (c) MSC sample M10 karyotype 46,XX. (d) MSC sample M12 karyotype 46,XY. (e) CNV profiles of three MSC cell lines showing the results observed by CMA. Microarray probes are plotted along the -axis according to their genomic coordinates and each color represents a chromosome (indicated below), from 1 to 22, and (from the short to the long arm). The -axis represents the log² scale of the copy number ratio MSC cell lines/control (values close to 0 indicate regions with similar copy number between tested and control samples; positive values represent gains; negative values represent losses). The arrow shows the detected trisomy of chromosome 5 in mosaic.

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