Preclinical Evaluation of the Tumorigenic and Immunomodulatory Properties of Human Bone Marrow Mesenchymal Stromal Cell Populations with Clonal Trisomy 5

Figure 2

Characterization of human MSC cell cultures. (a) Cumulative population doublings during cellular expansion ex vivo. Passage interval of samples: M10: P5 to P10; M12: P7 to P12; T5-50: P6 to P8; T5-53: P7 to P10. (b) Percentage of proliferative cells. One-Way ANOVA and Tukey’s multiple comparisons test. (c) Percentage of cells with SA-β-galactosidase activity; 100 cells analyzed per well. One-Way ANOVA and Tukey’s multiple comparisons test. (d) Intensity of SA-β-galactosidase activity per cell; cell fluorescence intensity (CF) calculated with ImageJ by . Kruskal-Wallis and Dunn’s multiple comparisons test. (e) Comparison of cellular morphology of M10 (Passage 8) and M12 (Passage 10) with T5-50 (Passage 7) and T5-53 (Passage 8) samples. T5-50 and T5-53 cells with typical flattened and enlarged senescent morphology. All pictures were captured using 100x magnification. Assays were performed in triplicate, at the same passage. Error bars represent standard deviation. value < 0.001; value < 0.0001; ns: statistically nonsignificant. M10 and M12: euploid human bone marrow MSC samples; T5-50 and T5-53: human bone marrow MSC samples with clonal trisomy 5.

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