Endothelial Cells Promote Migration of Mesenchymal Stem Cells via PDGF-BB/PDGFRβ-Src-Akt in the Context of Inflammatory Microenvironment upon Bone Defect

Figure 2

Src and Akt were required for ECs-induced MSCs migration in inflammatory microenvironment. (a) Representative images of migrated hBMSCs in Transwell culture systems. Pathway inhibitors were used for pre-treating cells migrating to IEC-CM. The quantification of migrated cells was shown as a bar graph. Data were compared with the groups of IEC-CM and shPDGFRβ from Figure 1. Scale bar, 50 μm. . (b) Representative images of wound healing assays. The rate of scratch wound closure was shown as a bar graph. Data were compared with the groups of IEC-CM and shPDGFRβ from Figure 1. Scale bar, 200 μm. . IEC-CM: conditioned media of endothelial cells in the context of inflammatory microenvironment; shPDGFRβ: short hairpin RNA targeting pdgfrb; shSrc: shRNA targeting src; shAkt: shRNA targeting akt.

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