Endothelial Cells Promote Migration of Mesenchymal Stem Cells via PDGF-BB/PDGFRβ-Src-Akt in the Context of Inflammatory Microenvironment upon Bone Defect

Figure 4

Data of in vivo experiments. (a) Fluorescence-activated cell sorting of CD31⁺ cells within implants at postoperative days 1, 3, and 7. The percent of CD31⁺ cells migrated cells was shown as a bar graph. (b) Gene and protein expression of PDGF-BB in sorted CD31⁺ cells. Data were shown as bar graphs. (c) A positive correlation lay between the ratio of CD31⁺cells and the protein level of PDGF-BB (Pearson’s correlation coefficient  =0.926, ). (d) Representative images of infiltrated CD31⁺ cells and PDGF-BB levels within implants. The quantitative comparison was detailed as bar graphs ( =5). (e) Representative images of homed MSCs within implants. The quantitative comparisons were detailed as bar graphs ( =5). Scale bars, 10 μm. . Triangles, implants. Arrows, staining positive cells. shPDGFRβ: short hairpin RNA targeting pdgfrb; shSrc: shRNA targeting src; shAkt: shRNA targeting akt.

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