Endothelial Cells Promote Migration of Mesenchymal Stem Cells via PDGF-BB/PDGFRβ-Src-Akt in the Context of Inflammatory Microenvironment upon Bone Defect

Table 3

Antibody information.

Antibody	Usage	Host-reactivity	Dilutions	Clonality	Source
PDGF-BB	Western blot	Rabbit anti-mouse	1 : 500	Polyclonal	Abcam, Cambridge, UK
PDGF-BB	Western blot	Chicken anti-human	1 : 500	Polyclonal	Abcam, Cambridge, UK
PDGFRβ	Western blot	Rabbit anti-mouse/human	1 : 1000	Monoclonal	Cell Signaling Technology, Danvers, MA, USA
Src	Western blot	Rabbit anti-mouse/human	1 : 1000	Monoclonal	Cell Signaling Technology, Danvers, MA, USA
p-Src	Western blot	Rabbit anti-mouse/human	1 : 1000	Monoclonal	Cell Signaling Technology, Danvers, MA, USA
Akt	Western blot	Rabbit anti-mouse/human	1 : 1000	Monoclonal	Cell Signaling Technology, Danvers, MA, USA
p-Akt	Western blot	Rabbit anti-mouse/human	1 : 2000	Monoclonal	Cell Signaling Technology, Danvers, MA, USA
CD31	Immunofluorescence	Goat anti-mouse	1 : 100	Polyclonal	R&D Systems, Minneapolis, MN, USA
PDGFRβ	Immunofluorescence	Goat anti-mouse	1 : 100	Polyclonal	R&D Systems, Minneapolis, MN, USA
Anti-GFP	Immunofluorescence	Rabbit anti-GFP	1 : 200	Polyclonal	Abcam, Cambridge, UK
Alexa Fluor® 488	Immunofluorescence	Goat anti-rabbit	1 : 200	Polyclonal	Abcam, Cambridge, UK
NL557	Immunofluorescence	Donkey anti-goat	1 : 500	Polyclonal	R&D Systems, Minneapolis, MN, USA