Cell Surface Proteins for Enrichment and In Vitro Characterization of Human Pluripotent Stem Cell-Derived Myogenic Progenitors

Figure 2

Selection of potential surface markers to enrich human PSC-derived myogenic progenitors. (a) 33 antibodies among the 89 antibodies stained positive in FGF-2-treated cells showed noticeable different expression levels in nontreated cells: 10 showed higher expression in FGF-2-treated cells and 23 showed lower expression in FGF-2-treated cells. (b) FGF-2-treated cells stained positive for CD29 and CD56 but negative for CD6 and CD83. Pink = positive for the surface marker indicated; blue = all nuclei. ; enlarged image . (c) Higher CD271 expression was seen in FGF-2 treated cells, whereas CD15 expression was higher in nontreated cells. ; enlarged image . (d) CD9 and CD15 fit our criteria of selection that 30-70% of myogenic progenitor culture was stained positive with high intensity and clear morphology; CD61, CD49a, and CD95 did not fit the criteria. ; .

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