Cell Surface Proteins for Enrichment and In Vitro Characterization of Human Pluripotent Stem Cell-Derived Myogenic Progenitors

Figure 3

Myotube-forming efficiency of human iPSC-derived myogenic progenitors enriched using identified markers. (a–c) Representative ICC images of MyHC expression (stained green) in differentiated cultures of human iPSC-derived myogenic progenitors sorted using MACS. . (d) Means of % MyHC⁺ cells in differentiated cultures of unsorted, positive, and negative fractions. Error bars represent SEM from three independent experiments. Statistical significance was calculated by one-way ANOVA followed by Fisher’s LSD post hoc test. , , and . (e) Means of % MyHC⁺ cells of sorted fractions normalized by the % MyHC⁺ cells of parallel unsorted fractions. Error bars represent SEM from three independent experiments. Statistical significance was calculated by one-way ANOVA followed by Fisher’s LSD post hoc test. , significantly different compared to unsorted fractions.

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