Cell Surface Proteins for Enrichment and In Vitro Characterization of Human Pluripotent Stem Cell-Derived Myogenic Progenitors

Figure 4

Characterization of myotubes formed by human iPSC-derived myogenic progenitors enriched using CD9 and CD146. (a) Representative ICC images of MyHC expression (stained green) in differentiated cultures of sorted human iPSC-derived myogenic progenitors. . (b–d) Properties of myotubes formed by differentiated cultures of unsorted cells and CD9⁺ and CD9^– human iPSC-derived myogenic progenitors: means of myotube width in microns, means of % myotube with ≥3 nuclei, and means of % myotube with 1 to ≥6 nuclei. (e–g) Properties of myotubes formed by differentiated cultures of unsorted and CD146⁺ and CD146^– human iPSC-derived myogenic progenitors: means of myotube width in microns, means of % myotube with ≥3 nuclei, and means of % myotube with 1 to ≥6 nuclei. Error bars represent SEM from three independent experiments. Statistical significance was calculated by one-way ANOVA followed by Fisher’s LSD post hoc test. , .

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