Cell Surface Proteins for Enrichment and In Vitro Characterization of Human Pluripotent Stem Cell-Derived Myogenic Progenitors

Figure 6

Purification of human iPSC-derived myogenic progenitors altered culture resilience to cryopreservation and expansion. (a) Measurements and formulas to calculate volume of each sphere. (b) Growth rate of sphere cultures prepared from cells in unsorted, CD271⁺, and CD15^– fractions after cryopreservation and culture expansion, presented as relative change in volume over 9 days in expansion medium. Error bars represent SEM from three independent experiments. Values on day 9 were analyzed by one-way ANOVA followed by Dunnett’s post hoc test. . (c) Means of % MyHC⁺ cells in differentiated cultures prepared from unsorted, CD271⁺, and CD15^– spheres after cryopreservation and expansion. Error bars represent SEM from three independent experiments. No statistical significance as calculated by one-way ANOVA. (d) Representative ICC images of MyHC expression (stained green) corresponding to (c). ; .

(a)

(b)

(c)

(d)