Cell Surface Proteins for Enrichment and <i>In Vitro</i> Characterization of Human Pluripotent Stem Cell-Derived Myogenic Progenitors

<div>Knockdown of CD271 in human PSC-derived myogenic progenitors reduced and impaired myotube-forming ability. (a) Western blot analysis showed successful knockdown of CD271 levels after treatment with CD271 siRNA #1 and #2 compared to scrambled as a negative control. This antibody clone might recognize another posttranslational fragment, hence the faint bands in the CD271 blotting. Beta-actin served as the loading control. (b) Means of % MyHC<sup>+</sup> cells in differentiated cultures prepared from cells treated with CD271 siRNA scrambled (negative control) #1 or #2. Error bars represent SEM from 6 analyzed images. Statistical significance was calculated by one-way ANOVA followed by Dunnett’s post hoc test. <span class="nowrap"><svg height="13.532pt" id="M33" style="vertical-align:-3.4295pt" version="1.1" viewbox="-0.0498162 -10.1025 68.0304 13.532" width="68.0304pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 144C444 143 470 148 486 158Z"></path></g><g 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109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg>.</span> (c) Representative immunocytochemistry images of MyHC expression (stained green) corresponding to (b). <span class="nowrap"><svg height="12.7373pt" id="M34" style="vertical-align:-3.429399pt" version="1.1" viewbox="-0.0498162 -9.3079 93.5425 12.7373" width="93.5425pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M409 504C401 567 396 607 392 642C354 654 312 665 266 665C137 665 60 583 60 487C60 374 161 325 225 290C300 250 355 215 355 141C355 68 311 21 235 21C131 21 86 122 71 183L41 176C48 128 61 42 68 21C78 16 93 8 118 0C142 -7 175 -15 216 -15C349 -15 438 69 438 174C438 287 344 333 265 374C186 414 138 449 138 522C138 576 172 631 249 631C336 631 363 562 380 499L409 504Z"></path></g><g transform="matrix(.013,0,0,-0.013,6.345,0)"><path d="M390 111C344 68 312 56 269 56C212 56 118 102 118 241C118 346 175 401 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Stem Cells International

fig7

Figure 7

Figure 7: Cell Surface Proteins for Enrichment and <i>In Vitro</i> Characterization of Human Pluripotent Stem Cell-Derived Myogenic Progenitors 

Stem Cells International

Cell Surface Proteins for Enrichment and In Vitro Characterization of Human Pluripotent Stem Cell-Derived Myogenic Progenitors

Figure 7