E3 Ubiquitin Ligase CHIP Inhibits the Interaction between Hsp90β and MAST1 to Repress Radiation Resistance in Non-Small-Cell Lung Cancer Stem Cells

Figure 2

Expression of the E3 ubiquitin ligase CHIP in NSCLC stem cells. (a) Flow cytometric sorting of CD133⁺ cells from the cancer tissues of NSCLC patients () and the lung cancer cell line A549. The isolated CD133⁺ cells were stained with anti-CD133⁺ antibody. (b) Representative images of sphere formation after 7 days of sphere culture of CD133^- and CD133⁺ NSCLC and A549 cells. (c) The expression of stemness-related transcription factors (Oct4, SOX2, and Nanog) in CD133^- and CD133⁺ NSCLC and A549 cells detected by RT-qPCR. (d) Clonal survival rate quantitation of radiation-exposed CD133^- and CD133⁺ NSCLC and A549 cells. (e) Apoptosis of CD133^- and CD133⁺ NSCLC and A549 cells detected by Annexin V/PI double staining. (f) The CD133 expression in CD133^- and CD133⁺ NSCLC and A549 cells detected by RT-qPCR. vs. CD133^- cells. Cell experiments were independently repeated three times.

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