Generation of Hepatocytes and Nonparenchymal Cell Codifferentiation System from Human-Induced Pluripotent Stem Cells

Figure 3

Hepatocyte functional assay after hepatocyte and nonparenchymal cell codifferentiation. (a) Quantitative PCR analysis showed the expression levels of mature hepatocyte markers. , , , and . (b, c) Immunofluorescence showed the coexpression of mature hepatocyte markers in day 25 cells. Scale bars, 50 μm. , , , and . (d, e) Hepatocytes performed PAS staining (top), ORO staining (middle), and ICG uptake/release analysis (bottom). Scale bars, 25 μm. , , , and . (f, g) Phase-contrast images of senescence β-Gal staining in hepatic cells during days 35-45 treated with Wnt3a or BMP4. Scale bars, 25 μm. , , , and . (h) Production of ALB and AAT in day 35 hepatocytes. Primary hepatocytes were used as positive controls; hiPSCs were used as negative control. , , , and .

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