FBXW7 Reduces the Cancer Stem Cell-Like Properties of Hepatocellular Carcinoma by Regulating the Ubiquitination and Degradation of ACTL6A

Figure 1

FBXW7 overexpression inhibits the stem cell-like properties of HCC cells. (a) FBXW7 expression was detected in spheres and their adherent HCC cells by RT-qPCR and Western blot. (b) RT-qPCR and Western blot analysis of mRNA and protein level of FBXW7 in the cell lines infected with LV-FBXW7 and LV-Vector. (c, d) CD133 surface marker expression was analyzed in the two cell lines by flow cytometry and IF staining. (e) Representative images and quantitative analysis of spheres. Scale bar: 100 mm. (f, g) Pluripotent transcription factors, including Nanog, SOX2, OCT4, and c-Myc, were analyzed by q-PCR (f) and Western blot (g). (h) Flow cytometry showed the effect of FBXW7 on the cell cycle in MHCC-97H and Huh7 cells. (i) Western blot analysis of cyclin E1 and p21 after FBXW7 alternation in MHCC-97H and Huh7 cells. Graph represents ; , , .

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