FBXW7 Reduces the Cancer Stem Cell-Like Properties of Hepatocellular Carcinoma by Regulating the Ubiquitination and Degradation of ACTL6A

Figure 4

E3 ubiquitin ligase FBXW7 interacts with ACTL6A. (a) Cell extracts from MHCC-97 cells with Flag-FBXW7 expression were immunopurified on an anti-Flag affinity column and eluted with Flag peptides. The eluates were analyzed by mass spectrometry. (b) Predictive analysis of E3 ubiquitin ligases that might target ACTL6A was performed using UbiBrowser database. (c) Co-IP and immunoblotting detected the interaction between FBXW7 and ACTL6A. (d) IF staining of FBXW7 and ACTL6A in the above cells. (e) Western blot was used to detect the effect of FBXW7 on the ACTL6A protein level. (f, g) Comparison of the expression of pluripotency-associated markers in HCC cells of different groups by Western blot. (h, i) Self-renewal abilities were measured by sphere formation assays in MHCC-97H and Huh7 cells transfected with LV-ACTL6A or shACTL6A#2. Graph represents ; , .

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