FBXW7 Reduces the Cancer Stem Cell-Like Properties of Hepatocellular Carcinoma by Regulating the Ubiquitination and Degradation of ACTL6A

Figure 5

FBXW7 negatively regulates ACTL6A stability via the ubiquitin-proteasome pathway. (a) RT-qPCR analysis of ACTL6A transcript level in MHCC-97H and Huh7 cells. (b) The above cells were incubated with MG132 (10 μM) for 12 h, and total protein was extracted and subjected to Western blot. (c–f) MHCC-97H and Huh7 cells were treated with CHX (50 μg/mL), harvested, and immunoblotted for ACTL6A and GAPDH. Quantification of the ACTL6A protein levels relative to GAPDH expression. Graph represents ; , .

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