Extracellular Vesicles of Mesenchymal Stem Cells Are More Effectively Accessed through Polyethylene Glycol-Based Precipitation than by Ultracentrifugation

Figure 1

(a) Comparison of the quantity of EVs harvested by UC or PEG. (b) AF-MSCs, which are sources of EV production; . EVs derived by UC and PEG presented similar morphology by electron microscopy, . (c) The proteins of EVs were quantified by BCA, and more proteins were derived by PEG than by UC (, for ). (d) The distribution of EV sizes derived by UC or PEG. (e) There was no difference in the diameter of the particles (), (f) while significantly more particles were harvested by PEG than by UC (, for ), (g) and there was no difference in particles per μg protein between UC-EVs and PEG-EVs.

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