Extracellular Vesicles of Mesenchymal Stem Cells Are More Effectively Accessed through Polyethylene Glycol-Based Precipitation than by Ultracentrifugation

Figure 2

Comparison of RNA expression of UC-EVs and PEG-EVs. (a) The copy numbers of RNY1, RNY3, RNY4, RNY5, miR-146a-5p, and Let-7b-5p detected in EVs by droplet digital PCR; blue points indicate positive reads, and gray points indicate negative reads. (b) the statistical analysis of RNA expression (). The concrete level of each RNA marker was statistically analyzed by the positive copy number reads of droplet digital PCR and its loading quantity. The RNA level of each gene was different by an order of magnitude; the most highly expressed was RNY3, and no Let-7b-5p signal was detected. There was a significant increase in the detected RNA of UC-derived EVs compared with PEG, for .

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