Extracellular Vesicles of Mesenchymal Stem Cells Are More Effectively Accessed through Polyethylene Glycol-Based Precipitation than by Ultracentrifugation

Figure 5

EV treatment leads to changes in the protein expression of THP-1 cells with LPS induction. (a) The in situ CD11b and CD163 expression of THP-1 cells was analyzed by immunofluorescence staining, . There were strong positive cells (white arrow), medium positive cells (yellow arrow), and negative cells (red arrow) in each group. The statistical analysis of MFI (mean fluorescence intensity) indicated that there were no significant differences in (b) CD11b and (c) CD163 in the groups. (d) Western blotting was used to determine the protein levels of phospho-P65, total-p65, and iNOS, and GAPDH was used as a control. The statistical analysis of protein levels indicated that iNOS relative to GAPDH was significantly decreased by EV treatment (# compared with the LPS group and ), but there were no significant differences in (e) P-P65 relative to T-P65 and (f) iNOS relative to GAPDH in comparison to UC-EVs and PEG-EVs.

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