Chidamide Suppresses the Growth of Cholangiocarcinoma by Inhibiting HDAC3 and Promoting FOXO1 Acetylation

Figure 4

CDM enhances FOXO1 acetylation by inhibiting HDAC3. (a) HDAC3 protein expression and FOXO1 acetylation level in QBC939 and SNU308 cells treated with sh-HDAC3 detected by Western blotting. and , compared with cells treated with sh-NC. (b) After inhibiting the expression of HDAC3 in QBC939 and SNU308 cells, IP was performed by FLAG precipitation and nuclear and cytosolic Ac-FOXO1. (c) HDAC3 protein expression and FOXO1 acetylation level in QBC939 and SNU308 cells treated with CDM-H or combined with oe-HDAC3 detected by Western blotting. , compared with cells treated with CDM-H. ^#, compared with cells treated with oe-NC. (d) After overexpressing HDAC3 in QBC939 and SNU308 cells, IP was performed by FLAG precipitation and nuclear and cytosolic Ac-FOXO1. Data were shown as the . An unpaired -test was employed for data comparison between two groups, while one-way ANOVA with Tukey’s post hoc test was used for multi-group data comparison. The experiment was repeated three times.

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