Macrophages with Different Polarization Phenotypes Influence Cementoblast Mineralization through Exosomes

Figure 4

Macrophages with different polarization phenotypes influenced cementoblast mineralization through exosomes. (a) Representative pictures of the exosomes observed by transmission electron microscopy. White arrows indicate the representative exosomes. Scale bars: 100 nm. (b) Representative chemiluminescent images of ALIX, CD63, and GM130 western blots from exosomes. (c) The mRNA expression levels of BSP, COL-1, RUNX2, OSX, and PTPLA in cementoblasts after a 3-day culture with exosomes from M0, M1, or M2 (referred to as M0-exo, M1-exo, and M2-exo). (d) Representative chemiluminescent images of BSP, COL-1, RUNX2, and OSX western blots from cementoblasts after a 3-day culture with exosomes from M0, M1, or M2 (referred to as M0-exo, M1-exo, and M2-exo). (e) Western blot analysis of BSP, COL-1, RUNX2, and OSX. The gray value was calibrated using β-actin. , , , and . (f) Confocal microscopy of OCN with DAPI counterstaining in cementoblasts after a 3-day culture with exosomes from M0, M1, or M2 (referred to as M0-exo, M1-exo, and M2-exo). Scale bars: 100 μm. (g) ALP staining of cementoblasts after a 5-day culture with exosomes from M0, M1, or M2(referred to as M0-exo, M1-exo, and M2-exo).

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