Peptidoglycan-Mediated Bone Marrow Autonomic Neuropathy Impairs Hematopoietic Stem/Progenitor Cells via a NOD1-Dependent Pathway in db/db Mice

Figure 3

Ex vivo administration of PGN did not alter the vasoreparative functions or colony-forming ability of diabetic LK cells. (a, b) LK cells were isolated from db/m and db/db mice and treated with either PGN or vehicle ex vivo ( per group). (a) Ex vivo PGN treatment did not further blunt the migration of LK cells from diabetic mice to the chemoattractant CXCL12 by the fluorometric QCM 5 μM96-well chemotaxis cell migration assay. (b) PGN-treated LK cells also showed similar proliferation ability measured by the proliferation BrdU ELISA kit in vitro. (c) CFU assay was used to determine the colony-forming abilities of BM and blood cells. The ex vivo administration of PGN did not alter the colony-forming abilities of BM and blood cells from diabetic mice ( per group). Data represent . PGN: peptidoglycan; V: vehicle. , ; ^&compared to db/m+V, ^#compared to db/m+PGN.

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