Schwann Cells Accelerate Osteogenesis via the Mif/CD74/FOXO1 Signaling Pathway In Vitro

Figure 1

(a) Diagram of the Schwann cells and MC3T3-E1 cell coculture model; (b) representative images showing ALP staining; (c) ALP activity was measured with an ALP assay kit, ; l; (d) representative images showing Alizarin red staining; l; (e) real-time RT-PCR (qPCR) analyses. , Student’s -test. ; (f–i) transcriptome differences between the coculture group and the control group in MC3T3-E1 cells; (f) the boxplots represent the distribution of expression levels; the ordinate is gene expression level; (g) volcano plot of differential gene expression analysis; the dotted blue line indicates the threshold of the differential genes selected; (h) cluster heatmap of differentially expressed genes; (i) the KEGG enrichment scatter plot. The size of the black spots represents the gene number; the gradual color represents the value.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)