An Alternate Approach to Generate Induced Pluripotent Stem Cells with Precise CRISPR/Cas9 Tool

Figure 3

Proliferative and immunological validation. (a, b) Cell viability of HEK293T-OSK and HEK293T (a) as well as HDF-OSK and HDF (b) were measured by MTT assay. (c) Supernatant was collected from HDF-OSK and HDF cells and analyzed for the amount of secreted interleukin-(IL-)6 with respective ELISA kit. The absorbance was measured by Synergy™ HTX multimode microplate reader. The statistical comparison among variables was done by multiway analysis of variance (ANOVA) with Tukey’s test in PRISM (GraphPad Software, San Diego, CA, USA) statistical software. The values <0.05 (), <0.01 (), and <0.001 () were considered statistically significant. The used immunostimulatory ligands included rhTNF-α (1 ng/ml), rhIL-1β (50 ng/ml), lipopolysaccharide (LPS; 100 ng/ml), and polyinosinic: polycytidylic acid (pIC; 1 μg/ml). ns = nonsignificant.

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