An Alternate Approach to Generate Induced Pluripotent Stem Cells with Precise CRISPR/Cas9 Tool

Figure 4

Generation and characterization of the iPSCs. (a) Workflow for the generation of iPSCs from HDF-OSK and HEK293T-OSK cells using a reprogramming medium. (b) Morphological changes during the reprogramming of HDF-OSK and HEK293T-OSK cells into iPSCs followed by their expansion in a maintenance medium. Scale bar: 100 μm. (c) Expression levels of pluripotency markers in the generated iPSC clones in comparison with human embryonic stem cells (hESCs). (d and e) Immunofluorescence assays of pluripotency markers in the iPSC clones derived from HDF-OSK cells (d) or HEK293T-OSK cells (e). Scale bar: 50 μm. (f) Silencing of GFP expression in morphologically changed HDF-OSK clones observed by fluorescence microscopy. Scale bar: 200 μm.

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