An Alternate Approach to Generate Induced Pluripotent Stem Cells with Precise CRISPR/Cas9 Tool

Figure 5

The differentiation potential of iPSCs. (a) Formation of embryoid bodies (black arrowhead) by the hanging drop method. Scale bar: 100 μm. (b and c) Immunofluorescence staining of spontaneously differentiating embryoid bodies of HDF-OSK (b) or HEK293T-OSK (c) clones for markers of each germ layer, i.e., α-fetoprotein (AFP) for endoderm, α-smooth muscle actin (α-SMA) for mesoderm, and β III tubulin (TUJ-1) for ectoderm. Nuclei were stained with a Hoechst 33258 solution, and images were captured by means of a fluorescence microscope (Olympus IX53; Olympus Corporation, Tokyo, Japan). Scale bar: 25 μm.

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