Xeno-Free Integrated Platform for Robust Production of Cardiomyocyte Sheets from hiPSCs

Figure 4

Structural and calcium transient analysis of hiPSC-derived cardiomyocytes. (a) Analysis of hiPSC-CM cardiac fibers organization at day 30 by confocal microscopy. A mixed population was observed. (b) Thin Filament length (cTnT bands) measured at day 30. Violin plot displays median and quartiles, cardiomyocytes from two independent experiments. (c) Calcium transients measured at day 20 showed a similar average duration for all differentiation conditions. Error bars, SEM: Control, Control, P&L, and replating, replating and P&L (ANOVA). (d) Representative calcium transient profile for replating, showing an average time between transients of 9.3, . (e) When 1 μM isoproterenol was supplemented, arrhythmic behavior was observed for a few samples, with some cardiac sheets showing arrested contractibility and calcium transients. Figure shows an average time between calcium transients of 11.6, . (f) When 1 μM carbachol was used, calcium transients showed a more consistent and synchronized pattern. Figure shows an average time between calcium transients of 6.9, .

(a)

(b)

(c)

(d)

(e)

(f)