Wharton’s Jelly-Derived Mesenchymal Stem Cells with High Aurora Kinase A Expression Show Improved Proliferation, Migration, and Therapeutic Potential

Figure 2

Knockdown of AURKA and DOCK2 inhibited cell proliferation, colony formation, and migration of WJ-MSCs. (a) Proliferation was evaluated by cell counting and CCK-8 assay at 0, 24, 72, and 144 h after seeding the siRNA-transfected cells. (b) Colony formation assays were performed in triplicate, with 1,000 cells per well to start with. Colonies were stained and counted two weeks after plating. (c) Migration was examined by wound healing assay. Knockdown of AURKA and DOCK2 inhibited the migration of WJ-MSCs. (d) Downregulation of AURKA inhibited phosphorylation of AKT and FAK while that of DOCK2 inhibited phosphorylation of AKT, ERK, and JNK. Normalized protein level indicates the value converted based on the siNC group. Data are shown as . One-way ANOVA with Dunnett’s multiple comparison test (b–d), and Tukey’s multiple comparisons test (a). , , and .

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