Wharton’s Jelly-Derived Mesenchymal Stem Cells with High Aurora Kinase A Expression Show Improved Proliferation, Migration, and Therapeutic Potential

Figure 3

Correlation between doubling time and mRNA expression of AURKA and DOCK2 in WJ-MSCs. (a) The graph shows doubling time in ten lots of WJ-MSCs. (b) Real-time qRT-PCR analysis showed mRNA expression levels of AURKA and DOCK2 in WJ-MSCs. Relative mRNA expression indicates the value converted based on that of MSC_J. Different letters above bars mean significant difference from others (one-way ANOVA followed by Tukey’s multiple range test, ). (c) Pearson’s correlation analysis confirmed a strong negative relationship between mRNA expression levels of AURKA and doubling time across the lots of WJ-MSCs. mRNA expression levels of DOCK2 and doubling time of WJ-MSCs showed a moderate negative relationship. (d) Western blotting of the protein levels of AURKA and DOCK2 in three WJ-MSCs. (e) Western blotting of the phosphorylation level of AKT, ERK, FAK, and JNK in three WJ-MSCs. Normalized protein level indicates the value converted based on that of MSC_A. Data are shown as . One-way ANOVA with Tukey’s multiple comparisons test (a, b) and Dunnett’s multiple comparisons test (d, e). , , and .

(a)

(b)

(c)

(d)

(e)