Wharton’s Jelly-Derived Mesenchymal Stem Cells with High Aurora Kinase A Expression Show Improved Proliferation, Migration, and Therapeutic Potential

Figure 5

Knockdown of AURKA inhibited antiapoptotic effect of WJ-MSCs and was accompanied by the reduction of XCL1 protein. (a) Western blotting of the protein expression levels of cleaved caspase-3 and cleaved PARP in apoptosis-induced C2C12 cells. Normalized protein level indicates the value converted based on that of the control group. (b) Representative fluorescence images showed whether the suppression of AURKA and DOCK2 inhibited antiapoptotic effects of WJ-MSC in C2C12 (green, apoptotic cells; blue, viable cells). (c) Western blotting of the expression level of XCL1 protein in siAURKA-transfected WJ-MSCs. Normalized protein level indicates the value converted based on that of the siNC group. Data are shown as . One-way ANOVA with Tukey’s multiple comparisons test (a, b). Two-tailed Student’s -test (c). , , and .

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