Research Article
Aucubin Impeded Preosteoclast Fusion and Enhanced CD31hi EMCNhi Vessel Angiogenesis in Ovariectomized Mice
Figure 3
Aucubin increases the number of preosteoclasts in vitro. (a) Cell viability of RAW264.7 cells cultured with different concentrations of aucubin (0, 1 μM, and 5 μM) was analyzed via a CCK-8 assay. (b–e) Relative mRNA expression levels of NFATc1, c-FOS, DC-STAMP, and ATP6V0D2 were analyzed. (f) Representative images of TRAP staining on day 6. Scale bar: 200 μm. (g) Quantification of osteoclasts (OCs) and preosteoclasts (POCs) on day 6. (h–j) Protein levels of c-Fos and NFATc1 were analyzed by WBs. (k) IF staining was performed to observe the location of NFATc1. Scale bar: 50 μm. (l) The quantification of the relative NFATc1 fluorescence intensity in different groups. compared to the control group; # compared to the Rankl group.
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