Aucubin Impeded Preosteoclast Fusion and Enhanced CD31^hi EMCN^hi Vessel Angiogenesis in Ovariectomized Mice

Figure 5

Aucubin promotes the proangiogenic effects of preosteoclasts on MMECs. (a) Representative images of the migration area of MMECs in different treatments. Scale bar: 200 μm. (b) The quantification of the migration area of MMECs in different treatments. Scale bar: 200 μm. (c) Representative images of tube formation in MMECs stimulated with different treatments. Scale bar: 200 μm. (d) The quantification of tube formation in MMECs stimulated with different treatments. (e) Relative mRNA expression levels of VEGF, MMP-9, and MMP-2 in MMECs with different CMs. (f) Concentration of VEGF in CM was analyzed by ELISA. (g) Representative images of the migration area of MMECs in different treatments. Scale bar: 200 μm. (h) The quantification of the migration area of MMECs in different treatments. compared to the vehicle+IgG group; ^& compared to the Rankl+IgG group; ^# compared to the Rankl+Aucubin+IgG group. (i) Representative images of tube formation in MMECs stimulated with different treatments. Scale bar: 200 μm. (h) The quantification of tube formation in MMECs stimulated with different treatments. compared to the vehicle+IgG group; ^& compared to the Rankl+IgG group; ^# compared to the Rankl+Aucubin+IgG group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)